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Anal Chem. 2013 Oct 15;85(20):9939-46. doi: 10.1021/ac402615c. Epub 2013 Sep 24. Simultaneous fluorescence imaging of the activities of DNases and 3' exonucleases in living cells with chimeric oligonucleotide probes.

Oligonucleotide probe

Authors E W Alm 1 An allele-specific oligonucleotide ( ASO) is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay. It is a common tool used in genetic testing, forensics, and Molecular Biology research. Oligonucleotide probe-ligation assays use ligases to join two oligo probes together when they are hybridized adjacently on a nucleic acid template molecule. We have developed OligoArray 2.0, a program that designs specific oligonucleotides at the genomic scale. It uses a thermodynamic approach to predict secondary structures and to calculate the specificity of targets on chips for a unique probe in a mixture of labeled probes. Light-activated (“caged”) oligonucleotides provide a strategy for modulating the activity of antisense oligos, siRNA, miRNA, aptamers, DNAzymes, and mRNA-capturing probes with high spatiotemporal resolution.

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The fluorescently labelled organisms can … Oligonucleotide Probe. ASO probes are generally short oligonucleotides (15–17mers) with a low GC content (or guanine-cytosine content) (usually 30–50%), designed such that the discriminating nucleotide is located somewhere in the middle of the probe. From: Molecular Diagnostics (Third Edition), 2017.

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Sequence-Specific Oligonucleotide (SSO) probes. are used in many important hybridization techniques including DNA microarrays designed for large-scale variation analysis and genotyping of complex DNA samples.

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probeBase—an online resource for rRNA-targeted oligonucleotide probes and primers: new features 2016. Nucleic Acids Res. 10.1093/nar/gkv1232 Full text probeBase by Daniel Greuter, Alexander Loy, Matthias Horn, and Thomas Rattei, Department of Microbiology and Ecosystem Science , University of Vienna Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure ().The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence. TaqMan probes consist of a fluorophore covalently attached to the 5’-end of the oligonucleotide probe and a quencher at the 3’-end. Several different fluorophores (e.g. 6-carboxyfluorescein, acronym: FAM, or tetrachlorofluorescein, acronym: TET) and quenchers (e.g. tetramethylrhodamine, acronym: TAMRA) are available.

The final paper, which is a regular paper, describes a stochastic pi-calculus model of the PHO pathway. Extensive set of mitochondrial LSU rDNA-based oligonucleotide probes for the detection of common airborne fungi. FEMS Microbiology Letters, Vol. 237, (1) Our group has pioneered methods such as oligonucleotide ligation assays, padlock, selector, and proximity probes, as well as the novel nFold and ExCirc The in situ hybridization-AT tailing (ISH-AT) method uses a specially designed and synthesized oligonucleotide probe that has (AT)(10) on the 3′ side allmän Nucleic Acid Probes. Nukleinsyratester. Svensk definition. Nukleinsyra som komplementerar en specifik mRNA- eller DNA-molekyl eller något fragment av [ÅK 7-9]aptamer selex and oligonucleotide probes. debbieevans: Medlem.
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doi: 10.1021/ac402615c. Epub 2013 Sep 24. Simultaneous fluorescence imaging of the activities of DNases and 3' exonucleases in living cells with chimeric oligonucleotide probes. Reducing the oligonucleotide length will also enhance the oligonucleotide specificity by conferring a larger destabilizing effect on mismatches between the target and non‐specific probes. One of the first steps of sequence processing is to mask prohibited sequences selected by users. A label-free bifunctional colorimetric oligonucleotide probe for DNA and protein detection has been developed on the basis of a novel catalytic molecular beacon consisting of two hairpin structures and a split G-quadruplex DNAzyme in the middle. The two loops of this molecular beacon consist of thrombin aptamer sequence and the complementary sequence of target DNA, which are utilized to sense Each oligonucleotide probe entry contains information on target organisms, target molecule (small- or large-subunit rRNA) and position, G+C content, predicted melting temperature, molecular weight, necessity of competitor probes, and the reference that originally described the oligonucleotide probe, including a link to the respective abstract at PubMed.

An important application of fluorogenic oligonucleotide probes is detection of DNA and RNA in both live and fixed cells. 35 A common approach is to use 2′-O-methyl oligoribonucleotide probes (2′-OMe-ORN) instead of DNA probes, as the former are much more stable in live cells, and the latter can also cause degradation of the target mRNA via the action of endogenous RNase H. 13,36 2′-OMe Oligonucleotide Probes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more The combination of PCR technology and hybridization with sequence-specific oligonucleotide probes was first applied to HLA class II typing because of the limitations of DR serology and of the better knowledge of allelic polymorphism at DR/DQ loci.
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These probes are usually 15 to 45 bases of single-stranded NA that are chemically synthesized as a specified base sequence. Most commonly, they are DNA, but RNA or NA analogs can also be synthesized. Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics. Commonly made in the laboratory by solid-phase chemical synthesis, these small bits of nucleic acids can be manufactured as single-stranded molecules with any user-specified sequence, and so are vital for artificial gene synthesis, polymerase chain reaction, DNA sequencing, molecular cloning and as molecular probes. In nature, oligonucleotides are usuall The oligonucleotide probe is labeled to a specific activity of 109 cpm/ μ g with [ γ - 32 P]ATP by the reaction catalyzed by T 4 kinase. This probe is used to screen 5 × 10 5 clones from rat heart library. Positive clones are purified through three more cycles of screening.

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TaqMan probes consist of a fluorophore covalently attached to the 5’-end of the oligonucleotide probe and a quencher at the 3’-end. Several different fluorophores (e.g. 6-carboxyfluorescein, acronym: FAM, or tetrachlorofluorescein, acronym: TET) and quenchers (e.g. tetramethylrhodamine, acronym: TAMRA) are available. Oligonucleotide probe design--a new approach The use of oligonucleotides for gene amplification, as diagnostic probes or antisense-based drugs can be optimized by minimizing the possibility of nonspecific hybridization. Oligonucleotide Probe Branched-Chain Amino Acids, Part B Gene Probes Neuropeptide Technology Oligonucleotide Probe. ASO probes are generally short oligonucleotides (15–17mers) with a low GC content (or guanine-cytosine content) (usually 30–50%), designed such that the discriminating nucleotide is located somewhere in the middle of the probe.